Comparative transcriptome analysis of twoCercospora sojinastrains reveals differences in virulence under nitrogen starvation stress

文献类型： 外文期刊

第一作者： Gu, Xin

作者： Gu, Xin;Yang, Xiaohe;Yao, Liangliang;Gao, Xuedong;Zhang, Maoming;Liu, Wei;Zhao, Haihong;Wang, Qingsheng;Li, Zengjie;Li, Zhimin;Ding, Junjie;Yang, Shuai

作者机构：

关键词： Cercospora sojina; RNA-Seq; Comparative transcriptomics; Nitrogen starvation; Weighted gene co-expression network

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Cercospora sojinais a fungal pathogen that causes frogeye leaf spot in soybean-producing regions, leading to severe yield losses worldwide. It exhibits variations in virulence due to race differentiation between strains. However, the candidate virulence-related genes are unknown because the infection process is slow, making it difficult to collect transcriptome samples. Results In this study, virulence-related differentially expressed genes (DEGs) were obtained from the highly virulent Race 15 strain and mildly virulent Race1 strain under nitrogen starvation stress, which mimics the physiology of the pathogen during infection. Weighted gene co-expression network analysis (WGCNA) was then used to find co-expressed gene modules and assess the relationship between gene networks and phenotypes. Upon comparison of the transcriptomic differences in virulence between the strains, a total of 378 and 124 DEGs were upregulated, while 294 and 220 were downregulated in Race 1 and Race 15, respectively. Annotation of these DEGs revealed that many were associated with virulence differences, including scytalone dehydratase, 1,3,8-trihydroxynaphthalene reductase, and beta-1,3-glucanase. In addition, two modules highly correlated with the highly virulent strain Race 15 and 36 virulence-related DEGs were found to contain mostly beta-1,4-glucanase, beta-1,4-xylanas, and cellobiose dehydrogenase. Conclusions These important nitrogen starvation-responsive DEGs are frequently involved in the synthesis of melanin, polyphosphate storage in the vacuole, lignocellulose degradation, and cellulose degradation during fungal development and differentiation. Transcriptome analysis indicated unique gene expression patterns, providing further insight into pathogenesis.

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