Stacking triple genes increased proanthocyanidins level inArabidopsis thaliana

文献类型： 外文期刊

第一作者： Wei, Jiebing

作者： Wei, Jiebing;Yang, Junfeng;Wei, Jiebing;Yang, Junfeng;Wei, Jiebing;Yang, Junfeng;Jiang, Wenbo;Pang, Yongzhen;Wei, Jiebing;Yang, Junfeng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Anthocyanins and proanthocyanidins are two important plant secondary metabolites, and they contribute to plant survival and human health. In particular, proanthocyanidins could also prevent ruminants from the damage of pasture bloat. However, the improvement of proanthocyanidins content remain unsatisfied. In this study, we attempted to improve proanthocyanidins level by gene stacking inArabidopsis thalianaas prove-of-concept. Two proanthocyanidin pathway genes from tea plant,CsF3'5'HandCsANR2, were co-expressed in the wild type andPAP1over-expressionArabidopsis. Over-expression ofCsF3'5'Hslightly affected anthocyanins level in leaves and proanthocyanidins in mature seed when expressed alone in thepap1-Dline. Over-expression ofCsANR2led to an obvious decrease in anthocyanins in leaves of both wild type andpap1-Dlines, but increase in proanthocyanidin level in mature seeds. Over-expression ofCsANR2inpap1-Dlines lead to production of DMACA-reactive soluble proanthocyanidins in leaves, but not in wild type orpap1-D lines. Anthocyanins level was decreased in the leaves ofCsF3'5'H,CsANR2andpap1-Dco-expression lines, but proanthocyanidins were increased remarkably in both leaves and mature seeds in the co-expression line. It is concluded that co-expression ofCsANR2andPAP1inArabidopsisproduce soluble proanthocyanidins in leaves, and co-expression ofCsF3'5'H,CsANR2andPAP1lead to a significant increase in proanthocyanidins in mature seeds. The transcript levels of endogenousCHS,DFR,ANSandANRgenes inArabidopsiswere up-regulated in the triple genes co-expression line. Based on these studies, it is possible to develop new plant germplasm with improved proanthocyanidins by co-expressing of multiple genes.

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