Comparative proteomics analysis indicates that palmatine contributes to transepithelial migration by regulating cellular adhesion

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Yang, Feng;Xin, Ruihua;He, Jiongjie;Cui, Dongan;Sun, Yan;Zhang, Shidong

作者机构：

关键词： Proteomics; mucosal inflammation; cell adhesion molecules

期刊名称：PHARMACEUTICAL BIOLOGY （ 影响因子：3.503； 五年影响因子：3.594 ）

ISSN： 1388-0209

年卷期： 2020 年 58 卷 1 期

页码：

收录情况： SCI

摘要： Context Palmatine, a biologically active isoquinoline alkaloid, possesses multiple pharmaceutical activities against mucosal infection and inflammation. Objective There are no reports about the influence of palmatine on uterine mucosal epithelial cells. Materials and methods We used proteomics to analyse differentially expressed proteins (DEPs) in goat endometrial epithelial cells (EECs) stimulated by lipopolysaccharide (LPS, 5 mu g/mL, the dosage can induce inflammatory response, according to our previous study) for 12 h and then treated with palmatine (80 mu g/mL) for 8 h; the dosage was selected based on MTT assay. The EECs without any treatment were used as controls. Every group was treated in triplicate. Results A total of 428 DEPs in LPS-stimulated group and 486 DEPs in the palmatine-treated group were identified. Functional annotation analysis showed that palmatine mainly regulated the protein expression of structural molecules involved in the response to stimuli. Pathway analysis showed that cell adhesion molecule (CaM) pathways were most significant enriched due to palmatine treatment. Junction adhesion molecule 1 (JAM1), nectin 1 (NECT1) and cadherin 5 (CDH5), which play important roles in the transepithelial migration (TEpM) of leukocytes, were significantly downregulated by palmatine. Meanwhile, other proteins essential to the maintenance of cell adhesion and those that facilitate leukocyte migration were upregulated after palmatine treatment. Discussion and conclusions:The results suggested that palmatine regulates the expression of CaMs to affect TEpM during uterine mucosal inflammation and provides novel insight to understanding and developing palmatine pharmacology. Palmatine is a promising drug for treatment of mucosal inflammation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序