Isolation ofTSCD11Gene for Early Chloroplast Development under High Temperature in Rice

文献类型： 外文期刊

第一作者： Fang, Guonan

作者： Fang, Guonan;Yang, Shenglong;Ruan, Banpu;Liu, Chaolei;Zhang, Anpeng;Jiang, Hongzhen;Ding, Shilin;Tian, Biao;Zhang, Yu;Jahan, Noushin;Zhu, Li;Zhang, Guangheng;Dong, Guojun;Zhang, Qiang;Zeng, Dali;Guo, Longbiao;Gao, Zhenyu;Qian, Qian

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关键词： TSCD11; Seryl-tRNA synthetase; Temperature-sensitive; Chloroplast development; Rice

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2020 年 13 卷 1 期

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收录情况： SCI

摘要： Background Chloroplasts are essential for photosynthesis and play key roles in plant development. High temperature affects structure of chloroplasts and metabolism in plants. The seryl-tRNA synthetase plays an important role in translation of proteins. Although seryl-tRNA synthetase has been widely studied in microbes and animals, few studies have reported about its role in chloroplast development under high temperature in rice. Results In this study, we isolated a noveltemperature-sensitive chlorophyll-deficient 11(tscd11) mutant by ethyl methane sulfonate (EMS) mutagenesis ofjaponicavariety Wuyujing7. Thetscd11mutant developed albino leaves at the 3-leaf stage under high temperature (35 degrees C), but had normal green leaves under low temperature (25 degrees C). Consistent with the albino phenotype, impaired chloroplasts, decreased chlorophyll content and increased ROS accumulation were found in thetscd11mutant at 35 degrees C. Fine mapping and DNA sequencing oftscd11revealed a missense mutation (G to A) in the eighth exon ofLOC_Os11g39670resulted in amino acid change (Glu(374)to Lys(374)). TheTSCD11gene encodes a seryl-tRNA synthetase localized to chloroplast. Complementation test confirmed that the point mutation inTSCD11is responsible for the phenotype oftscd11.TSCD11is highly expressed in leaves. Compared with the wild type (WT), mutation inTSCD11led to significant alteration in expression levels of genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development under high temperature. Conclusions TSCD11, encoding a seryl-tRNA synthetase localized to chloroplast, is vital to early chloroplast development at high temperature in rice, which help to further study on the molecular mechanism of chloroplast development under high temperature.

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