Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein
文献类型： 外文期刊
第一作者： Yan, Miaomiao
作者： Yan, Miaomiao;Huang, Jingling;Chen, Jianing;Yang, Wenting;Liu, Guangliang
作者机构：
关键词： atypical porcine pestivirus; monoclonal antibodies; NS3 protein
期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）
ISSN： 1040-6387
年卷期： 2020 年 32 卷 5 期
页码：
收录情况： SCI
摘要： Atypical porcine pestivirus (APPV) had been detected in many countries. However, to date, a commercial detection kit is not available because of a lack of specific monoclonal antibodies (mAbs) to APPV. We generated 7 mAbs targeting the NS3 protein of APPV. Isotyping results indicated that all of these mAbs are IgG1 with a kappa light chain. We analyzed the epitopes recognized by mAbs 2B6, 6G11, 8D1, 8D3, and 8F12, which recognized the same linear epitope (GRIKSAYSDE); the 6H3 and 7E10 mAbs recognized 2 different conformational epitopes. Applications of these antibodies were verified by ELISA, western blot, indirect immunofluorescence assay, and flow cytometry. The antibodies were functionally workable for these immunoassays except for 8F12, which could not be used in flow cytometry.
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