Opsonic Activity of Conservative Versus Variable Regions of the Group A Streptococcus M Protein

文献类型： 外文期刊

第一作者： Dai, Chuankai

作者： Dai, Chuankai;Hussein, Waleed M.;Yang, Jieru;Wang, Xiumin;Zhao, Lili;Toth, Istvan;Stephenson, Rachel J.;Khalil, Zeinab G.;Capon, Robert J.;Toth, Istvan;Hussein, Waleed M.;Wang, Xiumin;Wang, Xiumin;Toth, Istvan

作者机构：

关键词： opsonization; M protein; J8-epitope; 88/30-epitope; Group A Streptococcus; peptide-based subunit vaccine

期刊名称：VACCINES （ 影响因子：4.422； 五年影响因子：5.513 ）

ISSN：

年卷期： 2020 年 8 卷 2 期

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收录情况： SCI

摘要： Group A Streptococcus (GAS) and GAS-associated infections are a global challenge, with no licensed GAS vaccine on the market. The GAS M protein is a critical virulence factor in the fight against GAS infection, and it has been a primary target for GAS vaccine development. Measuring functional opsonic antibodies against GAS is an important component in the clinical development path for effective vaccines. In this study, we compared the opsonic activity of two synthetic, self-adjuvanting subunit vaccines containing either the J8- or 88/30-epitope in Swiss outbred mice using intranasal administration. Following primary immunization and three boosts, sera were assessed for IgG activity using ELISA, and opsonization activity against seven randomly selected clinical isolates of GAS was measured. Vaccine constructs containing the conservative J8-epitope showed significant opsonic activity against six out of the seven GAS clinical isolates, while the vaccine containing the variable 88/30-epitope did not show any significant opsonic activity.

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