Molecular Diagnostics and Detection of Oomycetes on Fiber Crops

文献类型： 外文期刊

第一作者： Wang, Tuhong

作者： Wang, Tuhong;Gao, Chunsheng;Cheng, Yi;Li, Zhimin;Chen, Jia;Guo, Litao;Xu, Jianping;Wang, Tuhong;Gao, Chunsheng;Cheng, Yi;Li, Zhimin;Chen, Jia;Guo, Litao;Xu, Jianping;Xu, Jianping

作者机构：

关键词： fiber crops; oomycete; molecular identification; mitochondrial DNA; PCR assay

期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）

ISSN：

年卷期： 2020 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Fiber crops are an important group of economic plants. Traditionally cultivated for fiber, fiber crops have also become sources of other materials such as food, animal feed, cosmetics and medicine. Asia and America are the two main production areas of fiber crops in the world. However, oomycete diseases have become an important factor limiting their yield and quality, causing devastating consequences for the production of fiber crops in many regions. To effectively control oomycete pathogens and reduce their negative impacts on these crops, it is very important to have fast and accurate detection systems, especially in the early stages of infection. With the rapid development of molecular biology, the diagnosis of plant pathogens has progressed from relying on traditional morphological features to the increasing use of molecular methods. The objective of this paper was to review the current status of research on molecular diagnosis of oomycete pathogens on fiber crops. Our search of PubMed identified nearly 30 species or subspecies of oomycetes on fiber crops, among which the top three species were Phytophthora boehmeriae, Phytophthora nicotianae and Pythium ultimum. The gene regions that have been used for molecular identifications of these pathogens include the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene cluster, and genes coding for translation elongation factor 1 alpha (EF-1 alpha) and mitochondrial cytochrome c oxidase subunits I and II (Cox 1, Cox 2), etc. We summarize the molecular assays that have been used to identify these pathogens and discuss potential areas of future development for fast, specific, and accurate diagnosis of oomycetes on fiber crops.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序