An Overview on the Mechanisms and Applications of Enzyme Inhibition-Based Methods for Determination of Organophosphate and Carbamate Pesticides

文献类型： 外文期刊

第一作者： Cao, Jing

作者： Cao, Jing;Wang, Miao;Yu, He;She, Yongxin;Cao, Zhen;Wang, Jing;Cao, Jing;Wang, Miao;Yu, He;She, Yongxin;Cao, Zhen;Wang, Jing;Wang, Jing;Lao, Shuibing;Ye, Jiaming;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Hacimuftuoglu, Ahmet

作者机构：

关键词： organophosphate pesticides; carbamate pesticides; rapid detection methods; devices; enzyme sources; reactivation; identification

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2020 年 68 卷 28 期

页码：

收录情况： SCI

摘要： Acetylcholinesterase inactivating compounds, such as organophosphate (OP) and carbamate (CM) pesticides, are widely used in agriculture to ensure sustainable production of food and feed. As a consequence of their applications, they would result in neurotoxicity, even death. In this essence, the development of enzyme inhibition methods still shows great significance as rapid detection techniques for on-site large-scale screening of OPs and CMs. Initially, mechanisms and applications of various enzyme-inhibition-based methods and devices, including optical colorimetric assay, fluorometric assays, electrochemical biosensors, rapid test card, and microfluidic device, are highlighted in the present overview. Further, to enhance the enzyme sensitivity for detection; alternative enzyme sources or high yield enrichment methods (such as abzyme, artificial enzyme, and recombinant enzyme), as well as enzyme reactivation and identification, are also addressed in this comprehensive overview.

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