Copper Tolerance Mediated byFgAceAandFgCrpAinFusarium graminearum

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Jiang, Yichen;He, Dan;Fang, Xin;Xu, Jianhong;Lee, Yin-Won;Shi, Jianrong;Liu, Xin;Keller, Nancy P.;Liu, Xin;Fang, Xin;Xu, Jianhong;Shi, Jianrong;Jiang, Yichen;Lee, Yin-Won;Keller, Nancy P.

作者机构：

关键词： Fusarium graminearum; copper tolerance; FgAceA; copper transporters; metallothionein; virulence

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： All organisms must secure essential trace elements (e.g., Cu) for survival and reproduction. However, excess trace element accumulation in cells is highly toxic. The maintenance of copper (Cu) homeostasis has been extensively studied in mammals, bacteria, and yeast but not in plant pathogens. In this study, we investigated the molecular mechanisms of copper tolerance inFusarium graminearum, the important wheat head scab fungus. RNA-seq revealed induced expression of the P-type ATPase transporterFgCrpAand metallothionein (MT)FgCrdAafter excess Cu treatment. Deletion ofFgCrpAbut notFgCrdAresulted in reduced tolerance to Cu toxicity. The "Cu fist" transcription factor FgAceA was involved in Cu detoxification through activation ofFgCrpA. oFgAceA was more sensitive to copper toxicity than oFgCrpA and overexpression ofFgCrpArestored copper tolerance in oFgAceA.FgAceAnegatively regulated aurofusarin production and its biosynthetic gene expression. oFgCrpA and oFgAceA were reduced in virulence in flowering wheat heads and synthesized decreased amounts of the mycotoxin deoxynivalenol when challenged with excess Cu. Taken together, these results suggest that mediation of Cu tolerance inF. graminearummainly relies on the Cu efflux pump and thatFgAceAgoverns Cu detoxification through activation ofFgCrpA.

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