Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions
文献类型: 外文期刊
第一作者: Xiao, Yingping
作者: Xiao, Yingping;Qi, Phoebe X.;Wickham, Edward D.;Xiao, Yingping
作者机构:
关键词: Whey protein isolate (WPI); sugar beet pectin (SBP); Chemical composition; zeta-Potential; Emulsion stability
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN: 0308-8146
年卷期: 2020 年 330 卷
页码:
收录情况: SCI
摘要: We investigated changes in the chemical composition of WPI as a result of heating (60 degrees C, 72 h) with SBP in solution (pH 6.75). The concentration of WPI was kept at a constant (3%), whereas the level of SBP was varied at 1, 1.5, and 3%. The reaction products were examined using the Ellman's reagent, ninhydrin assay, and gel electrophoresis. The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration). The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, >= 1.5%, however, adversely affected the emulsion stability at neutral pH.
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