Chromosome Painting Based on Bulked Oligonucleotides in Cotton

文献类型： 外文期刊

第一作者： Liu, Yuling

作者： Liu, Yuling;Wang, Xiuyuan;Wei, Yangyang;Liu, Zhen;Lu, Quanwei;Peng, Renhai;Liu, Fang;Zhang, Tao

作者机构：

关键词： Gossypium raimondii; oligonucleotide; fluorescencein situhybridization; chromosome painting; 45S rDNA

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Chromosome painting is one of the key technologies in cytogenetic research, which can accurately identify chromosomes or chromosome regions. Oligonucleotide (oligo) probes designed based on genome sequences have both flexibility and specificity, which would be ideal probes for fluorescencein situhybridization (FISH) analysis of genome structure. In this study, the bulked oligos of the two arms of chromosome seven of cotton were developed based on the genome sequence ofGossypium raimondii(DD, 2n= 2x = 26), and each arm contains 12,544 oligos. Chromosome seven was easily identified in both D genome and AD genome cotton species using the bulked chromosome-specific painting probes. Together with 45S ribosomal DNA (rDNA) probe, the chromosome-specific painting probe was also successfully used to correct the chromosomal localization of 45S rDNA inG. raimondii. The study reveals that bulked oligos specific to a chromosome is a useful tool for chromosome painting in cotton.

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