A Novel alpha/beta Hydrolase Domain Protein Derived From Haemonchus contortus Acts at the Parasite-Host Interface

文献类型： 外文期刊

第一作者： Lu, Mingmin

作者： Lu, Mingmin;Tian, Xiaowei;Yan, Ruofeng;Xu, Lixin;Song, Xiaokai;Li, Xiangrui;Tian, Ai-Ling;Li, Charles

作者机构：

关键词： alpha/beta-hydrolase; H. contortus; excretory and secretory protein; immunomodulator; parasite-host interaction

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.561； 五年影响因子：7.624 ）

ISSN： 1664-3224

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The alpha/beta-hydrolase domain (ABHD) proteins belonging to alpha/beta-hydrolase (ABH) superfamily are ubiquitously distributed throughout all the organisms, and their functional roles have been implicated in energy metabolism, cell signaling, growth and development. In our preliminary work, we identified a novel ABHD protein derived fromHaemonchus contortusexcretory-secretory (ES) proteins (HcESPs) that interacted with host T cells. Here, we demonstrated thatH. contortusABHD (HcABHD) protein, expressed in all life-cycle stages ofH. contortus, is a mammalian ABHD17 homolog with immunodiagnostic utility and lipase activity. Given its catalytic activities and immunomodulatory potentials, we further investigated the functional diversity of HcABHD as an individual ES protein in parasite-host interactions. HcABHD protein may serve as depalmitoylase or thioesterase to suppress cell viability, inhibit cell proliferation, induce intrinsic and extrinsic T cell apoptosis, and cause cell cycle arrested at G1 phase. Moreover, recombinant HcABHD stimuli exerted critical controls on T cell cytokine production profiles, predominantly by inhibiting the secretions of interleukin (IL)-4, interferon-gamma (IFN-gamma) and transforming growth factor-beta (TGF-beta) 1, and promoting IL-10 production. As the immunomodulator acting at the parasite-host interface, HcABHD protein may have potential applications for the vaccine development of therapeutic intervention. Together, these findings may help illuminate the molecular and particularly immunomodulatory aspects of ES proteins and contribute to an enhanced understanding of parasite immune evasion inH. contortus-host biology.

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