Salt-Induced Stability of SR1/CAMTA3 mRNA Is Mediated by Reactive Oxygen Species and Requires the 3 ' End of Its Open Reading Frame

文献类型： 外文期刊

第一作者： Abdel-Hameed, Amira A. E.

作者： Abdel-Hameed, Amira A. E.;Prasad, Kasavajhala V. S. K.;Reddy, Anireddy S. N.;Abdel-Hameed, Amira A. E.;Prasad, Kasavajhala V. S. K.;Reddy, Anireddy S. N.;Abdel-Hameed, Amira A. E.;Jiang, Qiyan

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关键词： Arabidopsis; Deadenylation; Decapping; mRNA stability; NMD; Reactive oxygen species; Salt stress; SR1/CAMTA3

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2020 年 61 卷 4 期

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收录情况： SCI

摘要： Soil salinity, a prevalent abiotic stress, causes enormous losses in global crop yields annually. Previous studies have shown that salt stress-induced reprogramming of gene expression contributes to the survival of plants under this stress. However, mechanisms regulating gene expression in response to salt stress at the posttranscriptional level are not well understood. In this study, we show that salt stress increases the level of Signal Responsive 1 (SR1) mRNA, a member of signal-responsive Ca2+ /calmodulin-regulated transcription factors, by enhancing its stability. We present multiple lines of evidence indicating that reactive oxygen species generated by NADPH oxidase activity mediate salt-induced SR1 transcript stability. Using mutants impaired in either nonsense-mediated decay, XRN4 or mRNA decapping pathways, we show that neither the nonsense-mediated mRNA decay pathway, XRN4 nor the decapping of SR1 mRNA is required for its decay. We analyzed the salt-induced accumulation of eight truncated versions of the SR1 coding region (similar to 3 kb) in the sr1 mutant background. This analysis identified a 500-nt region at the 3' end of the SR1 coding region to be required for the salt-induced stability of SR7 mRNA. Potential mechanisms by which this region confers SR1 transcript stability in response to salt are discussed.

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