High-resolution temporal dynamic transcriptome landscape reveals aGhCAL-mediated flowering regulatory pathway in cotton (Gossypium hirsutumL.)

文献类型： 外文期刊

第一作者： Shuaishuai Cheng

作者： Shuaishuai Cheng;Pengyun Chen;Zhengzheng Su;Liang Ma;Pengbo Hao;Jingjing Zhang;Qiang Ma;Guoyuan Liu;Ji Liu;Hantao Wang;Hengling Wei;Shuxun Yu

作者机构： College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi, People’s Republic of China;Chinese Acad Agr Sci, Minist Agr, Cotton Inst, State Key Lab Cotton Biol,Key Lab Cotton Genet Im, Anyang, Peoples R China;State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang 455000, Henan, China;College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi, People’s Republic of China

关键词： Gossypium hirsutum; early maturity; flower bud differentiation; transcriptome; GhCAL

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 2020影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

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收录情况： SCI

摘要： The transition from vegetative to reproductive growth is very important for early maturity in cotton. However, the genetic control of this highly dynamic and complex developmental process remains unclear. A high-resolution tissue- and stage-specific transcriptome profile was generated from six developmental stages using 72 samples of two early-maturing and two late-maturing cotton varieties. The results of histological analysis of paraffin sections showed that flower bud differentiation occurred at the third true leaf stage (3TLS) in early-maturing varieties, but at the fifth true leaf stage (5TLS) in late-maturing varieties. Using pairwise comparison and weighted gene co-expression network analysis, 5312 differentially expressed genes were obtained, which were divided into 10 gene co-expression modules. In the MElightcyan module, 46 candidate genes regulating cotton flower bud differentiation were identified and expressed at the flower bud differentiation stage. A novel key regulatory gene related to flower bud differentiation,GhCAL, was identified in the MElightcyan module.Anti-GhCALtransgenic cotton plants exhibited late flower bud differentiation and flowering time. GhCAL formed heterodimers with GhAP1-A04/GhAGL6-D09 and regulated the expression ofGhAP1-A04andGhAGL6-D09.GhAP1-A04- andGhAGL6-D09-silenced plants also showed significant late flowering. Finally, we propose a new flowering regulatory pathway mediated byGhCAL. This study elucidated the molecular mechanism of cotton flowering regulation and provides good genetic resources for cotton early-maturing breeding.

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