A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular Adipogenesis

文献类型： 外文期刊

第一作者： Ren, Ling

作者： Ren, Ling;Li, Qian;Hu, Xin;Xing, Yishen;Wang, Yahui;Li, Junya;Zhang, Lupei;Hu, Xin;Yang, Qiyuan;Du, Min;Du, Min

作者机构：

关键词： bta-miR-210; bovine; progenitor cells; intramuscular adipogenesis; WISP2

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 6 期

页码：

收录情况： SCI

摘要： Intramuscular fat (IMF) is one of the major factors determining beef quality. IMF formation is influenced by multiple conditions including genetic background, age and nutrition. In our previous investigation, bta-miR-210 was found to be increased during adipogenesis using miRNA-seq. In this study, we validated the upregulation of bta-miR-210 in platelet-derived growth factor receptor alpha positive (PDGFR alpha(+)) progenitor cells during adipogenic differentiation in vitro. To investigate its role in adipogenesis, bta-miR-210 mimics were introduced into progenitor cells, which resulted in enhanced intracellular lipid accumulation. Accordingly, the expression of adipocyte-specific genes significantly increased in the bta-miR-210 mimic group compared to that in the negative control group (p< 0.01). Dual-luciferase reporter assays revealed thatWISP2is a target of bta-miR-210.WISP2knockdown enhanced adipogenesis. In conclusion, bta-miR-210 positively regulates the adipogenesis of PDGFR alpha(+)cells derived from bovine fetal muscle by targetingWISP2.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序