Mutagenesis of Aspergillus aculeatus by 60Co-gamma Irradiation for High Production of Potential ILs-Tolerant Cellulase

文献类型： 外文期刊

第一作者： Xi, Rui-Ping

作者： Xi, Rui-Ping;Qi, Ye-Qiang;Hu, Xiao-Mei;Zhang, Bi-Xian;He, Xin-Miao;Chen, He-Shu;He, Xin-Miao;Chen, He-Shu;Gao, Yun-Fei

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关键词： Lignocellulosic biomass; Cellulose; Mutagenesis; Saccharification

期刊名称：BIORESOURCES （ 影响因子：1.614； 五年影响因子：1.923 ）

ISSN： 1930-2126

年卷期： 2020 年 15 卷 3 期

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收录情况： SCI

摘要： Ionic liquids (ILs) are effective solvents for lignocellulose pretreatment. Enzymatic saccharification converts pretreated lignocelluloses into valuable products, and IL-tolerant cellulase improves the enzymatic efficiency and the reuse of ILs. In this study, a fungal strain with a relatively high cellulase production was isolated and identified as Aspergillus aculeatus G1-3. The high production of 6-glucosidase (1.943 U per mL), CMCase (1.303 U per mL), and FPase (0.165 U per mL) was obtained using corn stover as the carbon source and peptone as the nitrogen source. The results were obtained at pH 8.0 and 30 degrees C with an inoculation size of 3% (volume per volume) for 7 days. A mutant strain Aspergillus aculeatus P6 with 6-glucosidase (7.023 U per mL), CMCase (1.543 U per mL), and FPase (0.098 U per mL) was obtained by 60Co-gamma irradiation. The cellulase activity was measured at pH 5.0 and 60 degrees C for enzymatic hydrolysis. The cellulase from mutant strains was stable in different concentrations of 1-ethyl-3-methylimidazolium acetate. Enzymatic saccharification of the original corn stover and ILs-pretreated corn stover was successfully performed with high sugar yields. The mutant strains of Aspergillus aculeatus have great potential for their further application in the conversion of lignocellulosic biomass into biofuels.

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