Generation of Transgene-Free Semidwarf Maize Plants by Gene Editing ofGibberellin-Oxidase20-3Using CRISPR/Cas9
文献类型： 外文期刊
第一作者： Zhang, Jiaojiao
作者： Zhang, Jiaojiao;Zhang, Xiaofeng;Chen, Rongrong;Yang, Li;Fan, Kaijian;Liu, Yan;Wang, Guoying;Ren, Zhenjing;Liu, Yunjun
作者机构：
关键词： CRISPR; Cas9; gene-editing; gibberellin oxidase; semidwarf; transgene-free
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： The "green revolution" gene gibberellin oxidase contributes to the semidwarf phenotype, improving product and lodging resistance. Dissecting the function of GA biosynthetic genes would be helpful for dwarf maize breeding. In this study, we edited the maizeGA20ox3gene and generated semidwarf maize plants using CRISPR/Cas9 technology. Application of exogenous gibberellin can recover the dwarf phenotype, indicating that the mutants are gibberellin deficient. The contents of GA(12)and GA(53)were elevated in the mutants due to the disruption of GA20 oxidase, whereas the contents of other GA precursors (GA(15), GA(24), GA(9), GA(44), and GA(20)) were decreased in the mutants, and the accumulation of bioactive GA(1)and GA(4)was also decreased, contributing to the semidwarf phenotype. Transgene-free dwarf maize was selected from T-2-generation plants and might be useful for maize breeding in the future.
分类号：
- 相关文献
作者其他论文 更多>>
-
Structural insights into the mechanism of phosphate recognition and transport by XPR1
作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu
关键词：
-
Yes-associated protein 1 is essential for maintaining lactation via regulating mammary epithelial cell dynamics and secretion capacity
作者：Lv, Haimiao;Wu, Zihui;Lin, Yuxin;Liu, Miaoyu;Xu, Linghua;Wang, Xiaojie;Sun, Nan;Abdelrahman, Mohamed;Yang, Liguo;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Xu, Xiaoling;Liu, Yan;Abdel-Shafy, Hamdy;Abdelrahman, Mohamed;Alsaegh, Aiman A.;Ahmed, Ahmed Ezzat;Ahmed, Ahmed Ezzat;Yang, Liguo;Hua, Guohua
关键词：Lactation; Yes-associated protein1(YAP1); Prolactin; Melatonin; Mammary epithelial cells
-
Bacterial cellulose and photothermal material from biomass-based zero-waste biorefinery functionalized chitosan film for sustainable active food packaging
作者：Yang, Li;Sheng, Wenyang;Yang, Yichen;He, Kaiwen;Jiang, Guangyang;Tian, Yongqiang;Yang, Li;Sheng, Wenyang;Yang, Yichen;He, Kaiwen;Jiang, Guangyang;Tian, Yongqiang;Yang, Ying
关键词：Zero-waste biorefining; Bacterial cellulose; Photothermal material; Chitosan; Antibacterial activity; Distillers' grains
-
New Isocoumarins from an Endophytic Fungal Strain Diaporthe arengae M2 and their Antibacterial Activities
作者：Liu, De-Sui;Ma, Qing-Yun;Yang, Li;Xie, Qing-Yi;Dai, Hao-Fu;Zhao, You-Xing;Liu, De-Sui;Ma, Qing-Yun;Yang, Li;Xie, Qing-Yi;Dai, Hao-Fu;Zhao, You-Xing;Liu, De-Sui;Zhang, Jun-Feng;Wu, You-Gen
关键词：Endophytic fungi;
Diaporthe sp.; Isocoumarin derivatives; Antibacterial activity -
Synergistic use of stay-green traits and UAV multispectral information in improving maize yield estimation with the random forest regression algorithm
作者：Liu, Yuan;Meng, Lin;Nie, Chenwei;Liu, Yadong;Song, Yang;Jin, Xiuliang;Liu, Yuan;Fan, Kaijian;Meng, Lin;Nie, Chenwei;Liu, Yadong;Song, Yang;Jin, Xiuliang;Cheng, Minghan
关键词：UAV multispectral; Maize yield; Stay-Green Index (SGI); Machine learning; Remote sensing
-
A LTR retrotransposon insertion leads to leafy phenotype in maize by elevating ZmOM66 expression
作者：Du, Xuemei;Chen, Yan;Gao, Xinpeng;Wang, Xiaoli;Cui, Yu;Liu, Yunjun;Fu, Junjie;Wang, Guoying;Du, Xuemei;Xu, Zhuoyi;Lu, Jiawen;Zhang, Jie;He, Cheng;Huang, Liying;Guo, Wei;Cui, Yangbo;Ai, Junmin;Li, Li;Gu, Riliang;Wang, Jianhua
关键词：
-
Temperature-controllable antibacterial film mediated by a photoresponsive metal-polyphenol framework for long-term food preservation
作者：Jiang, Guangyang;Sheng, Wenyang;Yang, Li;Yang, Yichen;Tian, Yongqiang;Jiang, Guangyang;Sheng, Wenyang;Yang, Li;Yang, Yichen;Tian, Yongqiang;Tang, Tingting;Wang, Chenzhi
关键词：Cu-CGA nanoparticles; Photothermal; Antimicrobial activity; Food packaging; Cherry tomatoes preservation
