Natural variation and CRISPR/Cas9-mediated mutation inGmPRR37affect photoperiodic flowering and contribute to regional adaptation of soybean

文献类型： 外文期刊

第一作者： Wang, Liwei

作者： Wang, Liwei;Sun, Shi;Wu, Tingting;Liu, Luping;Sun, Xuegang;Cai, Yupeng;Jia, Hongchang;Yuan, Shan;Chen, Li;Jiang, Bingjun;Wu, Cunxiang;Hou, Wensheng;Han, Tianfu;Li, Jicun

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关键词： soybean (Glycine max(L; ) Merr; ); flowering time; QTL; GmPRR37; CRISPR; Cas9; adaptation

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2020 年 18 卷 9 期

页码：

收录情况： SCI

摘要： Flowering time is a critical determinant of the geographic distribution and regional adaptability of soybean (Glycine max) and is strongly regulated by photoperiod and temperature. In this study, quantitative trait locus (QTL) mapping and subsequent candidate gene analysis revealed thatGmPRR37, encoding a pseudo-response regulator protein, is responsible for the major QTLqFT12-2, which was identified from a population of 308 recombinant inbred lines (RILs) derived from a cross between a very late-flowering soybean cultivar, 'Zigongdongdou (ZGDD)', and an extremely early-flowering cultivar, 'Heihe27 (HH27)', in multiple environments. Comparative analysis of parental sequencing data confirmed that HH27 contains a non-sense mutation that causes the loss of the CCT domain in the GmPRR37 protein. CRISPR/Cas9-inducedGmprr37-ZGDD mutants in soybean exhibited early flowering under natural long-day (NLD) conditions. Overexpression ofGmPRR37significantly delayed the flowering of transgenic soybean plants compared with wild-type under long photoperiod conditions. In addition, both the knockout and overexpression ofGmPRR37in soybean showed no significant phenotypic alterations in flowering time under short-day (SD) conditions. Furthermore,GmPRR37down-regulated the expression of the flowering-promotingFThomologuesGmFT2aandGmFT5a, and up-regulated flowering-inhibitingFThomologueGmFT1aexpression under long-day (LD) conditions. We analysed haplotypes ofGmPRR37among 180 cultivars collected across China and found naturalGmprr37mutants flower earlier and enable soybean to be cultivated at higher latitudes. This study demonstrates thatGmPRR37controls soybean photoperiodic flowering and provides opportunities to breed optimized cultivars with adaptation to specific regions and farming systems.

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