Ecdysone Receptor (EcR) and Ultraspiracle Protein (USP) Genes FromConopomorpha sinensisBradley Eggs: Identification and Expression in Response to Insecticides

文献类型： 外文期刊

第一作者： Yao, Qiong

作者： Yao, Qiong;Xu, Shu;Dong, Yizhi;Quan, Linfa;Chen, Bingxu;Yao, Qiong

作者机构：

关键词： litchi fruit borer; EcR; USP; ovicide; biomarker

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）

ISSN： 1664-042X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Conopomorpha sinensisBradley (Lepidoptera: Gracilariidae) is the dominant insect pest of litchi (chinensisSonn.) and longan (Euphoria longanLour.) fruit trees. Management of this pest species is a challenging task due to its cryptic borer behavior. ControllingC. sinensisat the egg stage is the best alternative strategy to chemical control ofC. sinensisadults. However, thorough studies regarding the indirect and sublethal effects of chemicals on the different developmental stages ofC. sinensisare insufficient. In this study, the effect of some insecticides was evaluated onC. sinensiseggs. The ovicidal activity of chlorbenzuron, abamectin, chlorantraniliprole, and lambda-cyhalothrin was confirmed by morphological observation of the defects inC. sinensiseggs. Moreover, we characterized four essential ecdysone receptor proteins in insects [i.e., two isoform ecdysone receptors (EcR: CsEcRA. CsEcRB) and two isoform ultraspiracle proteins (USP:CsUSP1,CsUSP2)] fromC. sinensiseggs. TheCsEcRA,CsEcRB,CsUSP1, andCsUSP2genes consisted of 1521-, 1614-, 1410-, and 1236-bp open reading frames which encoded proteins of 506, 527, 469, and 413 amino acid residues, respectively. Furthermore, the embryonic differential responses ofCsEcRs,CsUSPs, and vitellogenin receptor (VgR: CsVgR) to insecticides were evaluated by qRT-PCR. Among the five tested genes,CsVgRandCsUSP1were the most sensitive to all the tested insecticides, with fold change of the expression diminished by 4.27-8.70 times compared with untreated control insects. The data suggests that these insecticidal compounds regulate the expression of these specific proteins, which might eventually lead to reduced viability ofC. sinensiseggs. We present here the first data providing molecular elucidation of ecdysone receptor genes and their differential responses to insecticides inC. sinensiseggs. Together with our previous report of insecticide sublethal effects on two reproduction-related genes inC. sinensisadults,CsVgRandCsUSP1seem to be appropriate molecular parameters for the evaluation of insecticide impact onC. sinensis. This study exemplifies the potential utility of transcriptional measurement of nuclear receptors as the molecular biomarkers for ecotoxicological evaluations of ovicidal impact of insecticides.

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