Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase

文献类型： 外文期刊

第一作者： Li, Xianjun

作者： Li, Xianjun;Wang, Junhuan;Wu, Wei;Jia, Yang;Fan, Shuanghu;Hlaing, Thet Su;Khokhar, Ibatsam;Yan, Yanchun

作者机构：

关键词： Aromatic oxyphenoxypropionic acid esters; Bioremediation; Co-metabolic biodegradation; Desert soil; Genome sequencing; Grassy weeds; Herbicide; Methylobacterium populi; Novel metabolites; Quizalofop-p-ethyl; alpha/beta hydrolase

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2020 年 46 卷

页码：

收录情况： SCI

摘要： Background: Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPE-degrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. Results: A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as Methylobacterium populi, which could degrade QPE with methanol by cometabolism. Ninety-seven percent of QPE (50mg/L) could be degraded within 72 h under optimum biodegradation condition of 35 degrees C and pH 8.0. The maximum degradation rate of QPE was 1.4 mg/L/h, and the strain YC-XJ1 exhibited some certain salinity tolerance. Two novel metabolites, 2-hydroxy-6-chloroquinoxaline and quinoxaline, were found by high-performance liquid chromatography/mass spectroscopy analysis. The metabolic pathway of QPE was predicted. The catalytic efficiency of strain YC-XJ1 toward different AOPPs herbicides in descending order was as follows: haloxyfop-p-methyl approximate to diclofop-methyl approximate to fluazifop-p-butyl N clodinafop-propargyl N cyhalofop-butyl N quizalofop-p-ethyl N fenoxaprop-p-ethyl N propaquizafop N quizalofop-p-tefuryl. The genome of strain YC-XJ1 was sequenced using a combination of PacBio RS II and Illumina platforms. According to the annotation result, one alpha/beta hydrolase gene was selected and named qpeh1, for which QPE-degrading function has obtained validation. Based on the phylogenetic analysis and multiple sequence alignment with other QPE-degrading esterases reported previously, the QPEH1 was clustered with esterase family V. Conclusion: M. populi YC-XJ1 could degrade QPE with a novel pathway, and the qpeh1 gene was identified as one of QPE-degrading esterase gene. (C) 2020 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序