Data-Independent Acquisition Proteomics Unravels the Effects of Iron Ions on Coronatine Synthesis inPseudomonas syringaepv.tomatoDC3000

文献类型： 外文期刊

第一作者： He, Yan

作者： He, Yan;Yu, Sha;Liu, Shaojin;Tian, Hao;Yu, Chunxin;Tan, Weiming;Li, Zhaohu;Jiang, Feng;Duan, Liusheng;He, Yan;Yu, Sha;Liu, Shaojin;Tian, Hao;Yu, Chunxin;Tan, Weiming;Li, Zhaohu;Duan, Liusheng;Zhang, Jie;Jiang, Feng

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关键词： proteomics; iron ions; coronatine; Pseudomonas syringae; secondary metabolites

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Coronatine (COR) is a new type of plant growth regulator that is produced byPseudomonas syringaepathovars and plays an important role in modulating plant growth, development, and tolerance to multiple stresses. However, the factors affecting COR production are not very clear. In this study, the effects of FeCl(3)on COR production were researched. The data-independent acquisition (DIA) approach, which is a proteomic quantitative analysis method, was applied to quantitatively trace COR production and proteomic changes inP. syringaepv.tomatoDC3000 under different FeCl(3)culture conditions. The results showed that COR production increased with the addition of FeCl(3)and that there was significant upregulation in the expression of proteins related to COR synthesis and regulation. In addition, FeCl(3)also affected the expression of related proteins involved in various metabolic pathways such as glycolysis and the tricarboxylic acid cycle. Moreover, various precursors such as isoleucine and succinate semialdehyde, as well as other related proteins involved in the COR synthesis pathway, were significantly differentially expressed. Our findings revealed the dynamic regulation of COR production in response to FeCl(3)at the protein level and showed the potential of using the DIA method to track the dynamic changes of theP. syringaepv.tomatoDC3000 proteome during COR production, providing an important reference for future research on the regulatory mechanism of COR biosynthesis and theoretical support for COR fermentation production.

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