Effect of diflubenzuron on the chitin biosynthesis pathway inConopomorpha sinensiseggs

文献类型： 外文期刊

第一作者： Yao, Qiong

作者： Yao, Qiong;Quan, Lin-Fa;Xu, Shu;Dong, Yi-Zhi;Li, Wen-Jing;Chen, Bing-Xu;Yao, Qiong

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关键词： Conopomorpha sinensis; chitin; diflubenzuron; ovicidal effect; transcriptomics

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

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收录情况： SCI

摘要： Conopomorpha sinensisis the dominant borer pest ofLitchi chinensis(litchi) andEuphoria longan(longan) in China. Control ofC. sinensisis difficult because of its cryptic life habit; thus, an effective ovicide could be beneficial. The larvicidal effects of diflubenzuron (DFB) have been documented in many insect pest species. Therefore, DFB might be a useful ovicide to controlC. sinensis. However, the detailed mode of action of DFB interference with insect molting and egg hatching is unclear. Thus, we studied alterations in expression of all genes potentially affected by DFB treatment using a transcriptome approach in 2-d-oldC. sinensiseggs. Clean reads were assembled to generate 203 455 unigenes and 440 558 transcripts. A total of 4625 differently expressed genes, which included 2670 up-regulated and 1955 down-regulated unigenes, were identified. Chitin binding and chitin metabolic processes were among the most significant enriched pathways according to Gene Ontology analyses. Most of the genes that encode enzymes involved in the chitin biosynthesis pathway were unaffected, whereas genes that presumably encode cuticle proteins were up-regulated. Furthermore, altered expression patterns of 10 genes involved in the chitin biosynthesis pathway ofC. sinensisembryos were observed in response to DFB treatment at different time points by quantitative reverse transcription polymerase chain reaction. We also observed abnormal development; there was reduced chitin content and modulated chitin distribution of newly hatched larvae, and altered egg hatching. Our findings illustrate an ovicidal effect of DFB onC. sinensis, and reveal more molecular consequences of DFB treatment on insects.

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