Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity

文献类型： 外文期刊

第一作者： Miao, Xiangyang

作者： Miao, Xiangyang;Luo, Qingmiao;Xie, Lingli;Zhao, Huijing;Qin, Xiaoyu

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关键词： Sheep; Epigenetics; Ovary; Methylation; Fecundity

期刊名称：REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY （ 影响因子：5.211； 五年影响因子：5.017 ）

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年卷期： 2020 年 18 卷 1 期

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收录情况： SCI

摘要： Background/aims Sheep are important livestock with variant ovulation rate and fertility. Dorset sheep is a typical breed with low prolificacy, whereas Small Tail Han sheep with FecB mutation (HanBB) have hyperprolificacy. Our previous studies have revealed the gene expression difference between the ovaries from Dorset and HanBB sheep contributes to the difference of fecundity, however, what leads to these gene expression difference remains unclear. DNA methylation, an important epigenetic process, plays a crucial role in gene expression regulation. Methods In the present study, we constructed a methylated DNA immunoprecipitation combined with high throughput sequencing (MeDIP-seq) strategy to investigate the differentially methylated genes between the Dorset and HanBB ovaries. Results Our findings suggest the genes involved in immune response, branched-chain amino acid metabolism, cell growth and cell junction were differentially methylated in or around the gene body regions. Conclusions These findings provide prospective insights on the epigenetic basis of sheep fecundity.

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[1]Impact of FecB Mutation on Ovarian DNA Methylome in Small-Tail Han Sheep. Xie, Lingli,Miao, Xiangyang,Luo, Qingmiao,Zhao, Huijing,Qin, Xiaoyu. 2023

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