Chloroplast SRP54s are Essential for Chloroplast Development in Rice

文献类型： 外文期刊

第一作者： Shi, Yongfeng

作者： Shi, Yongfeng;He, Yan;Lv, Xiangguang;Wei, Yanlin;Zhang, Xiaobo;Xu, Xia;Li, Liangjian;Wu, Jian-li;Lv, Xiangguang

作者机构：

关键词： Rice; Pale green leaf; Chloroplast; Signal recognition particle; Map-based cloning

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Background The chloroplast signal recognition particle 54 (cpSRP54) is known for targeting the light-harvesting complex proteins to thylakoids and plays a critical role for chloroplast development in Arabidopsis, but little is known in rice. Here, we reported two homologous cpSRP54s that affect chloroplast development and plant survival in rice. Results Two ricecpSRP54homologues,OscpSRP54aandOscpSRP54b, were identified in present study. The defectiveOscpSRP54a(LOC_Os11g05552) was responsible for the pale green leaf phenotype of the viablepale green leaf 14(pgl14) mutant. A single nucleotide substitution from G to A at the position 278, the first intron splicing site, was detected inLOC_Os11g05552inpgl14. The wild type allele could rescue the mutant phenotype. Knockout lines ofOscpSRP54b(LOC_Os11g05556) exhibited similar pale green phenotype topgl14with reduced chlorophyll contents and impaired chloroplast development, but showed apparently arrested-growth and died within 3 weeks. BothOscpSRP54aandOscpSRP54bwere constitutively expressed mainly in shoots and leaves at the vegetative growth stage. Subcellular location indicated that both OscpSRP54a and OscpSRP54b were chloroplast-localized. Both OscpSRP54a and OscpSRP54b were able to interact with OscpSRP43, respectively. The transcript level ofOscpSRP43was significantly reduced while the transcript level ofOscpSRP54bwas apparently increased inpgl14.In contrast, the transcript levels ofOscpSRP54a,OscpSRP43andOscpSRP54bwere all significantly decreased inOscpSRP54bknockout lines. Conclusion Our study demonstrated that both OscpSRP54a and OscpSRP54b were essential for normal chloroplast development by interacting with OscpSRP43 in rice. OscpSRP54a and OscpSRP54b might play distinct roles in transporting different chloroplast proteins into thylakoids through cpSRP-mediated pathway.

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