Activity-based ratiometric fluorescent small-molecule probe for endogenously monitoring neutrophil elastase in living cells

文献类型： 外文期刊

第一作者： Cao, Ting

作者： Cao, Ting;Zheng, Lei;Qian, Jing;Ma, Hong;Wang, Jiemin;Qin, Wenwu;Cao, Ting;Zheng, Lei;Qian, Jing;Ma, Hong;Wang, Jiemin;Qin, Wenwu;Teng, Zhidong;Guo, Huichen;Teng, Zhidong;Guo, Huichen

作者机构：

关键词： Neutrophil elastase; Fluorescent sensors; Ratiometric; Endogenous; Cells imaging

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN： 0003-2670

年卷期： 2020 年 1127 卷

页码：

收录情况： SCI

摘要： Neutrophil elastase (NE), a representative protease which is closely related to many diseases, acts an indispensable role in inflammatory diseases and clinical medicine. In this work, one activity-based non-peptide ratiometric fluorescent probe DCDF was designed with pentafluoropropionic anhydride as identification group. To our knowledge, this is the first probe capable of detecting NE in ratio. After the addition of the NE, the emission spectrum of DCDF has obvious bathochromic-shift phenomenon, and there is large Stokes shifts of -60 nm. Compared to only a few reported NE probes, DCDF is sensitive and selective and has very low detection limit (0-14 mu g/mL, DL = 30.8 ng/mL). A possible response mechanism was proposed and verified by HPLC and HRMS spectra. What's more, DCDF is capable of endogenous recognition imaging in biological cells without interference from other enzymes under the ratio signal. A549 and HeLa cells were used for endogenous cell imaging experiments of NE and the feasibility of DCDF for the specific detection of NE in cells was proved. This experimental result makes probe DCDF a very promising tool for the clinical diagnosis and treatment of NE related diseases. (C) 2020 Elsevier B.V. All rights reserved.

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