Os4BGlu14, a monolignol beta-Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice

文献类型： 外文期刊

第一作者： Ren, Rui-Juan

作者： Ren, Rui-Juan;Wang, Pei;Chen, Xi-Wen;Wang, Li-Na;Chen, De-Fu;Su, Jing-Ping;Sun, Lin-Jing;Sun, Yue

作者机构：

关键词： Accelerated aging; beta-Glucosidase; Rice; Seed longevity; Metabolites

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2020 年 104 卷 4-5 期

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收录情况： SCI

摘要： Key message Os4BGlu14, a monolignol beta-glucosidase, plays a negative role in seed longevity by affecting primary metabolism during seed development and aging. Seed longevity is a crucial trait in agriculture and in the conservation of germplasm resources.beta-Glucosidases (BGlus) are multifunctional enzymes that affect plant growth and their adaptation to the environment. The function of rice BGlus in seed longevity, however, remains unknown. We report here that Os4BGlu14, a rice beta-Glucosidase, negatively affected seed longevity during accelerated aging. Os4BGlu14 was highly expressed in rice embryos and induced by accelerated aging. Compared to the wild type, rice lines overexpressing Os4BGlu14 had significantly greater grain length, but smaller grain width and thickness. Overexpressing (OE) lines also showed lower starch but higher glucose contents. After accelerated aging treatment, OE lines displayed a significantly lower germination percentage than the wild type. Additionally, these lines had higher lignin accumulation before and after accelerated aging. Metabolome analysis detected 217 metabolites in untreated and aged rice seeds. Comparison of the differential metabolites between WT and OE5 revealed that ten key metabolites, four of which (e.g., uridine 5 '-diphosphoglucose-glucose, UDPG) were increased, while the other six (e.g.,gamma-aminobutyric acid and methionine) were decreased, might be the crucial factors that lead to seed deterioration. Further analysis confirmed higher UDPG levels and more severe programmed cell death in OE lines than in the wild type. Furthermore, OE lines presented a lower germination rate after abscisic acid and paclobutrazol treatment during germination, compared to the wild type. Our study provides a basis for understanding the function of Os4BGlu14 in seed longevity in rice.

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