Selective and stable elimination of endosymbionts from multiple-infected whiteflyBemisia tabaciby feeding on a cotton plant cultured in antibiotic solutions

文献类型： 外文期刊

第一作者： Zhao, Dong-Xiao

作者： Zhao, Dong-Xiao;Zhang, Zhi-Chun;Niu, Hong-Tao;Guo, Hui-Fang

作者机构：

关键词： antibiotic treatment; Bemisia tabaci; co-infections; endosymbionts; infection frequency

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2020 年 27 卷 5 期

页码：

收录情况： SCI

摘要： The maternally heritable endosymbiont provides many ecosystem functions. Antibiotic elimination of a specific symbiont and establishment of experimental host lines lacking certain symbionts enable the roles of a given symbiont to be explored. The whiteflyBemisia tabaci(Gennadius) in China harbors obligate symbiontPortierainfecting each individual, as well as facultative symbionts, such asHamiltonella,RickettsiaandCardinium, with co-infections occurring relatively frequently. So far no studies have evaluated the selectivity and efficacy of a specific symbiont elimination using antibiotics in whiteflies co-infected with different symbionts. Furthermore, no success has been achieved in establishing certain symbiont-freeB. tabacilines. In this study, we treatedHamiltonella-infectedB. tabaciline,Hamiltonella-Rickettsia-co-infected line andHamiltonella-Cardiniumco-infected line by feedingB. tabaciadults with cotton plants cultured in water containing rifampicin, ampicillin or a mixture of them, aiming to selectively curing symbiont infections and establishing stable symbiont-free lines. We found ampicillin selectively eliminatedCardiniumwithout affectingPortiera,HamiltonellaandRickettsia, although they coexisted in the same host body. Meanwhile, all of the symbionts considered in our study can be removed by rifampicin. The reduction of facultative symbionts occurred at a much quicker pace than obligate symbiontPortieraduring rifampicin treatment. Also, we measured the stability of symbiont elimination in whitefly successive generations and establishedRickettsia-infected andCardinium-infected lines which are absent in natural populations. Our results provide new protocols for selective elimination of symbionts co-existing in a host and establishment of different symbiont-infected host lines.

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