Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

文献类型： 外文期刊

第一作者： Sun, Linlin

作者： Sun, Linlin;Guo, Xiaojie;Zhang, Yu;Liu, Xinyu;Li, Xuesong;Tian, Yazhen;Man, Chaoxin;Jiang, Yujun;Tian, Wenli

作者机构：

关键词： Lactobacillus gasseri; probiotic characteristics; immunomodulatory activity; PI3K/Akt

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 9 期

页码：

收录情况： SCI

摘要： Lactobacillus gasseri JM1, a novel strain isolated from infant feces, exhibited common probiotic proper-ties such as high acid tolerance, bile salt tolerance, and adhesion to epithelial Caco-2 cells, suggesting its abil-ity to survive in the gastrointestinal tract and confer potential probiotic action on the host. In the current study, we aimed to evaluate the immunomodulatory activity of L. gasseri JM1 and explore the underlying signaling pathways in vitro. The results showed that pretreatment with L. gasseri JM1 alleviated lipopoly-saccharide-induced inflammatory response, as evi-denced by downregulation of genes encoding proinflam-matory cytokines [IL1B, IL6, IL8, and tumor necrosis factor-alpha (TNFA)] and upregulation of genes encoding anti-inflammatory cytokines [IL4, IL10, transforming growth factor-03 (TGFB3), and IFNG]. A high level of gene expression was noted for toll-like receptor 2 and NOD-like receptor 2. Meanwhile, transcriptomic sequencing obtained 84 differentially expressed genes. Kyoto Encyclopedia of Genes and Genomes analysis revealed the phosphatidylinositol 3-kinase (PI3K)/pro-tein kinase B (Akt) signaling pathway was activated by L. gasseri JM1 in Caco-2 cells. Inhibitor of PI3K/ Akt played various roles in the release of cytokines, indicating that the pathway was involved in protecting the host against lipopolysaccharide stress. Moreover, whole-genome sequencing revealed fundamental genetic properties of L. gasseri JM1 and provided clues for pro -biotic characteristics. In summary, the strain could ex-ert immunomodulatory effects via the toll-like receptor 2 and NOD2-mediated PI3K/Akt signaling pathway and be regarded as a potential probiotic.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序