Follicle Stimulating Hormone Inhibits the Expression of p53 Up-Regulated Modulator of Apoptosis Induced by Reactive Oxygen Species Through PI3K/Akt in Mouse Granulosa Cells

文献类型： 外文期刊

第一作者： Shi, Xiaoyan

作者： Shi, Xiaoyan;Shi, Xiaoyan;Guan, Zhiqiang;Liu, Honglin;Yu, Jianning

作者机构：

关键词： Follicle stimulating hormone; Oxidative stress; PI3K/AKT; Granulosa cells

期刊名称：PHYSIOLOGICAL RESEARCH （ 影响因子：1.881； 五年影响因子：2.199 ）

ISSN： 0862-8408

年卷期： 2020 年 69 卷 4 期

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收录情况： SCI

摘要： In mammalian ovaries, follicular atresia occurs periodically and destroys almost all the follicles in the ovary. Follicle-stimulating hormone (FSH) acts as the primary survival factor during follicular atresia by preventing apoptosis in granulosa cells (GCs). Many studies have demonstrated that oxidative stress-induced apoptosis is a main cause of follicular atresia. Reactive oxygen species (ROS)-induced GCs apoptosis is regulated by a variety of signaling pathways involving numerous genes and transcription factors. Therefore, we examined whether FSH inhibits the expression of p53 up-regulated modulator of apoptosis (PUMA) induced by reactive oxygen species (ROS) through phosphoinositide 3-kinase (PI3K) / protein kinase B (AKT) in mouse GCs. In vivo study: thirty-two-mice were randomly assigned to four groups and given FSH. We found that FSH can inhibit the 3-nitropropionic acid (3-NP) induced apoptosis and PUMA expression in mRNA level. Moreover, in vitro experiment, we found that FSH can inhibit the H2O2 -induced apoptosis and PUMA expression in mRNA level. Additionally, we also found that PI3K/AKT inhibitor LY294002 abolished the downregulation of PUMA mRNA by FSH in vitro. In conclusion, FSH inhibit the expression of PUMA induced by ROS through PI3K/AKT pathway in vivo and vitro.

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