Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

文献类型： 外文期刊

第一作者： Kong, Weiwei

作者： Kong, Weiwei;Wang, Liming;Ji, Jingjing;Dong, Puhui;Yan, Xuefang;Wang, Chunping;Cao, Pei;Li, Xingfeng;Wang, Honggang;Sun, Jiaqiang

作者机构：

关键词： Wheat; lmpa1; Mutant; Chromosomal location

期刊名称：BMC GENETICS （ 影响因子：2.797； 五年影响因子：3.263 ）

ISSN： 1471-2156

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Lesion-mimic and premature aging (lmpa) mutantlmpa1was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis oflmpa1mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out inlmpa1, KD527 and their F(1)and F(2)derivatives. Further, bulked segregation analysis (BSA) in combination with a 660 K SNP array were conducted on the F(2)segregation population oflmpa1/Chinese spring (CS) to locate thelmpa1gene. Results Most agronomic traits oflmpa1were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F(1)plants from the crossing oflmpa1and KD527 exhibited thelmpaphenotype and the F(2)progenies showed a segregation of normal (wild type, WT) andlmpa, with the ratios oflmpa: WT = 124:36(chi(2) = 1.008 < =3.841), indicating thatlmpais a dominant mutation. The combination of BSA and the SNP array analysis of CS,lmpa1andlmpa1/CS F2WT pool (50 plants) andlmpapool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging geneLmpa1was probably located on the short arm of chromosome 5A. Conclusions EMS-mutagenized mutantlmpa1 deriving from elite wheat line KD527 conferredlmpa.Lmpaphenotype oflmpa1 mutant is controlled by a single dominant allele designated asLmpa1, which affected wheat growth and development and reduced the thousand grain weight (tgw) of single plant in wheat. The geneLmpa1was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序