Over-Expression of a Transcription Factor GeneBoC3H4Enhances Salt Stress Tolerance but Reduces Sclerotinia Stem Rot Disease Resistance in Broccoli

文献类型： 外文期刊

第一作者： Jiang, Ming

作者： Jiang, Ming;Zhang, Huijuan;Zhu, Xin;Miao, Lixiang

作者机构：

关键词： Broccoli; Sclerotinia sclerotiorum; C3H zinc finger; Salinity stress

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

年卷期： 2020 年 39 卷 3 期

页码：

收录情况： SCI

摘要： C3H-type zinc finger genes play diverse roles in plant growth, development, and stress responses. In our present study, a C3H-type gene namelyBoC3H4was isolated from broccoli.BoC3H4was 1629 bp in length encoding 542 amino acid residues. The deduced protein sequence contained two ankyrin repeats and two CCCH zinc finger motifs, and those motifs shared high identities with homologous sequences from other Cruciferae plants. The expression levels ofBoC3H4elevated when subjected to both salt stress andSclerotinia sclerotioruminfection. Broccoli plants with constitutive expression ofBoC3H4demonstrated increased tolerance toward salinity stress, accompanied by a prominent accumulation of proline, and a remarkable decrease of chlorophyll loss, MDA, REC, as well as H(2)O(2)accumulation compared to WT plants. Moreover, over-expression ofBoC3H4in broccoli lines decreased resistance toS. sclerotiorum, and it could not induce the expression ofBoPDF1.2gene, the marker gene for JA/ET signaling pathway. Our study proposes thatBoC3H4acts as a positive regulator of plant tolerance to salinity stress and a negative regulator of resistance to necrotrophic pathogenS.sclerotiorum.

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