Metabolomics analysis of growth inhibition ofLactobacillus plantarumunder ethanol stress

文献类型： 外文期刊

第一作者： Chen, Xiaoqian

作者： Chen, Xiaoqian;Wang, Tingting;Tan, Ying;Liu, Libo;Li, Chun;Du, Peng;Jin, Man;Liu, Lihua;Yang, Yuzhuo

作者机构：

关键词： Ethanol stress; Lactobacillus plantarum; microbial scanning electron microscope; metabolomics; multivariate analysis

期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.713； 五年影响因子：3.408 ）

ISSN： 0950-5423

年卷期： 2020 年 55 卷 11 期

页码：

收录情况： SCI

摘要： This experiment investigated the changes in morphology, cell membrane permeability and intracellular metabolites ofLactobacillus plantarum(L. plantarum) under different concentrations of ethanol stress. It was found that when treated with ethanol at the concentration of 8% (v/v),L. plantarummortality was close to 60%, and the surface of cell was rough and collapsed, and the cell debris is obvious. The extracellular beta-galactosidase activity increased to 1.24 and 1.73 times higher as compared to the control group after 6% (v/v) and 8% (v/v) ethanol stress, respectively. The inhibition of bacterial growth was positively correlated with its concentration. Ethanol stress can cause changes in cell morphology and membrane permeability ofL. plantarum, causing physiological damage to cells. Metabolomics analysis revealed that the ethanol stress led to the inhibition of primary metabolic pathways through the suppression of the tricarboxylic acid cycle and glycolysis. It is therefore proposed that the inhibition effect of ethanol onL. plantarumis achieved by inducing intracellular metabolic imbalance via disruption of cell membrane functions. Importance Lactobacillus plantarumis often subjected to ethanol stress during food processing, production and storage, thus inhibiting the growth of bacteria. However, the detailed inhibition mechanism of ethanol onL. plantarumhas not been deeply studied on metabolomics analysis. By measuring the changes of intracellular metabolites under ethanol stress, we found that ethanol destroyed the function of the membrane by changing the membrane structure, which hindered the carbon metabolism in the cell centre and reduced the vitality of bacteria. The results made a contribution for improvement of the target strain physiological and the strain industrial utility under ethanol stress.

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