Reduced fecundity and regulation of reproductive factors in flubendiamide-resistant strains of Plutella xylostella

文献类型： 外文期刊

第一作者： Sun, Shi-Qing

作者： Sun, Shi-Qing;Wang, Nian-Meng;Li, Jing-Jing;Xue, Chao-Bin;Jin, Ming-Hui

作者机构：

关键词： Fecundity; Vitellin; Hormone; Trehalase; Flubendiamide-resistance

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2020 年 169 卷

页码：

收录情况： SCI

摘要： Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of flubendiamide has led to the development of resistance in field populations and reports of control failures. In this study, the lab-selected (R-f) and field-collected (R-b) flubendiamide-resistant strains of P. xylostella with LC50 resistance ratios of 1890-fold and 1251-fold, respectively, were used, as well as a lab-reared flubendiamidesusceptible strain (S). The results showed that the fecundity of the R-f and R-b -resistant strains was significantly lower than that of S strain. The contents of vitellin and transcripts of P. xylostella vitellogenin (PxVg) and P. xylostella vitellogenin receptor (PxVgR) genes in the R-f and R-b strains were significantly higher than those of S strains at 0-48 h after adult eclosion. At 96 h after eclosion, the content of vitellin in the R-f and R-b strains did not differ significantly from those of S strains, whereas transcripts of the PxVg and PxVgR genes in the R-f and R-b strains were significantly lower than that of the S strain. The content of the juvenile hormone III (JH III), beta-ecdysone (20E), and the gene expression level of P. xylostella methoprene tolerant (PxMet) in the R-f and R-b strains were significantly higher than that of the S strain. The activity of trehalase was significantly higher in the R-f and R-b strains than that of the S strain in the first to the third instar larvae, whereas in the fourth instar larvae, there was no significantly difference in the three strains. At different times after adult eclosion, the differences in trehalase activity were erratic between the strains. The transcripts of P. xylostella trehalase (PxTre) gene in the R-f and R-b strains were significantly higher than that of the S strain in most developmental stages. Here, we report differences in fecundity between flubendiamide-resistant and susceptible strains of P. xylostella and discuss gene expression of several reproductive factors, which provides a possible explanation for the mechanism of fecundity reduction concurrent with flubendiamide-resistance in P. xylostella.

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