Integrative omic and transgenic analyses reveal the positive effect of ultraviolet-B irradiation on salvianolic acid biosynthesis through upregulation ofSmNAC1

文献类型： 外文期刊

第一作者： Yin, Xiaojian

作者： Yin, Xiaojian;Fan, Hui;Song, Wei;Yin, Xiaojian;Chen, Yan;Li, Lan-Zhu;Fan, Yuanming;Zhou, Wei;Ma, Gaoxiang;Alolga, Raphael N.;Li, Ping;Lu, Xu;Qi, Lian-Wen;Li, Weiqiang;Zhang, Baolong;Tran, Lam-Son P.;Tran, Lam-Son P.

作者机构：

关键词： metabolomics; NAC1; proteomics; transcriptomics; Salvia miltiorrhiza; salvianolic acids; UV-B radiation

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2020 年 104 卷 3 期

页码：

收录情况： SCI

摘要： Salvianolic acids (SalAs), a group of secondary metabolites inSalvia miltiorrhiza, are widely used for treating cerebrovascular diseases. Their biosynthesis is modulated by a variety of abiotic factors, including ultraviolet-B (UV-B) irradiation; however, the underlying mechanisms remain largely unknown. Here, an integrated metabolomic, proteomic, and transcriptomic approach coupled with transgenic analyses was employed to dissect the mechanisms underlying UV-B irradiation-induced SalA biosynthesis. Results of metabolomics showed that 28 metabolites, including 12 SalAs, were elevated in leaves of UV-B-treatedS. miltiorrhiza. Meanwhile, the contents of several phytohormones, including jasmonic acid and salicylic acid, which positively modulate the biosynthesis of SalAs, also increased in UV-B-treatedS. miltiorrhiza. Consistently, 20 core biosynthetic enzymes and numerous transcription factors that are involved in SalA biosynthesis were elevated in treated samples as indicated by a comprehensive proteomic analysis. Correlation and gene expression analyses demonstrated that theNAC1gene, encoding a NAC transcriptional factor, was positively involved in UV-B-induced SalA biosynthesis. Accordingly, overexpression and RNA interference ofNAC1increased and decreased SalA contents, respectively, through regulation of key biosynthetic enzymes. Furthermore, ChIP-qPCR and Dual-LUC assays showed that NAC1 could directly bind to the CATGTG and CATGTC motifs present in the promoters of the SalA biosynthesis-related genesPAL3andTAT3, respectively, and activate their expression. Our results collectively demonstrate thatNAC1plays a crucial role in UV-B irradiation-induced SalA biosynthesis. Taken together, our findings provide mechanistic insights into the UV-B-induced SalA biosynthesis inS. miltiorrhiza, and shed light on a great potential for the development of SalA-abundant varieties through genetic engineering.

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