Fat mass- and obesity-associated (FTO) gene promoted myoblast differentiation through the focal adhesion pathway in chicken

文献类型： 外文期刊

第一作者： Huang, Huayun

作者： Huang, Huayun;Liu, Longzhou;Li, Chunmiao;Liang, Zhong;Huang, Zhenyang;Wang, Qianbao;Li, Shoufeng;Zhao, Zhenhua;Liu, Longzhou

作者机构：

关键词： FTO; Chicken; Myoblast proliferation; Myoblast differentiation; Focal adhesion

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2020 年 10 卷 9 期

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收录情况： SCI

摘要： The action ofFTOon myoblasts proliferation and differentiation and molecular mechanism underlying it were investigated by transfecting withFTOlentiviral overexpression vector and gene expression profile sequencing. Compared with the control group, myoblasts withFTOtransfection was significantly enhanced proliferation; the expression ofMYOGandMYOD mRNAwas significantly increased. In cells transfected withFTO, 129 differentially expressed genes were determined compared with control group, with 104 up-regulated and 25 down-regulated genes. Twelve pathways (Phagosome, Focal adhesion, Adrenergic signaling in cardiomyocytes, Endocytosis, Cardiac muscle contraction, Toll-like receptor, Ribosome, Tight junction, Regulation of actin cytoskeleton, Cytokine-cytokine receptor interaction, Adrenergic signaling in cardiomyocytes and MAPK) were significantly enriched. Eight genes known to be directly or indirectly related to skeletal muscle development (LAMA5,SPP1,CAV3,RASGRF1,FAK,PDGFB,PDGFR alpha,andRAC2) were enriched in the focal adhesion and expressed differentially. Altogether, these data suggested thatFTOstimulated differentiation of myoblasts through regulation of eight genes enriched in the focal adhesion.

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