Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry

文献类型： 外文期刊

第一作者： Xiang, Jian Jun

作者： Xiang, Jian Jun;Liao, Qie Gen;Yuan, Li Juan;Zhang, Da Wen;Xi, Ci Dan Zha

作者机构：

关键词： Azithromycin; Broiler feather; MCX@Fe3O4 nanoparticles; LC-MS/MS

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN： 1570-0232

年卷期： 2020 年 1152 卷

页码：

收录情况： SCI

摘要： In the study, a sensitive and reproducible method for the quantitative analysis of azithromycin in broiler feather samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. Feather samples were rinsed after being wrapped in medical gauze, then chopped and then added to 5% (v/v) ammonia in methanol solution for ultrasonic extraction. The extract was purified by the combination of commercial polymeric microparticles (Oasis MCX) and Fe3O4 nanoparticles. The LC separation was performed on an Agilent Eclipse plus C18 column. Multiple reaction monitoring was used for the selective detection of azithromycin. The good linearity curve of azithromycin in feather sample was in the range from 1.0 mu g kg(-1) to 100.0 mu g kg(-1) with 0.9935 of correlation coefficient. And the limit detection and limit of quantification was 0.5 mu g kg(-1) and 2.0 mu g kg(-1) in spiked feather samples. The recoveries of azithromycin were 85.2-94.7% with the relative standard deviation less than 10%. The established method is simple, rapid, sensitive and specific, and could meet the need of government and enterprises to monitor the illegal use of azithromycin in livestock and poultry breeding.

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