Multiplex PCR assay to detectAspergillus, PenicilliumandFusariumspecies simultaneously

文献类型： 外文期刊

第一作者： Rahman, Hamid Ur

作者： Rahman, Hamid Ur;Yue, Xiaofeng;Ren, Xianfeng;Zhang, Wen;Zhang, Qi;Li, Peiwu;Rahman, Hamid Ur;Yue, Xiaofeng;Zhang, Wen;Zhang, Qi;Li, Peiwu;Rahman, Hamid Ur;Ren, Xianfeng;Li, Peiwu;Yue, Xiaofeng;Zhang, Qi;Li, Peiwu;Zhang, Wen;Li, Peiwu;Li, Peiwu

作者机构：

关键词： Multiplex PCR; simultaneous detection; internal transcribed spacer; elongation factor 1 alpha

期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：3.057； 五年影响因子：2.96 ）

ISSN： 1944-0049

年卷期： 2020 年 37 卷 11 期

页码：

收录情况： SCI

摘要： A wide variety of mycotoxins is produced by mycotoxigenic fungi and naturally contaminates food and feed products worldwide. Synergistic effects of multi-toxins are potentially more harmful than exposure to a single compound and can induce acute and chronic toxicity to animals and humans. The aim of the present study is to timely and simultaneously identify the multiple mycotoxigenic fungi capable of causing synergistic toxicity to improve the safety level of food and feedstuff. Here, a multiplex polymerase chain reaction assay was developed for simultaneous detection of mycotoxigenic fungi belonging to the generaAspergillus, FusariumandPenicillium. Three pairs of genus-specific primers were designed based on internal transcribed spacer (ITS) sequences ofAspergillusandPenicillium, and Elongation factor 1 alpha (EF- 1 alpha) ofFusarium. Amplicons of 170, 750 and 490 bp respectively for the corresponding primer pairs were detected; thus amplicon length is diagnostic for the individual fungal genus. The sensitivity of the developed method was tested with genomic DNA obtained from mould pure cultures and artificially contaminated maize grain powder. The sensitivity result showed that spore concentrations in the contaminated maize grain powder of 10(2)spores/mL were detected without prior incubation. This result suggests that the developed mPCR assay would allow a rapid, specific and simultaneous detection of various mycotoxigenic potential fungi based on the occurrence and size of the amplification products and thus to estimate the multi-mycotoxins contamination potential in food and feedstuff.

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