Toxoplasmainvasion delayed byTgERK7 eradication

文献类型： 外文期刊

第一作者： Li, Zhong-Yuan

作者： Li, Zhong-Yuan;Liang, Xue;Guo, Hai-Ting;Tan, Jie;Zhu, Xing-Quan;Liu, Quan;Liu, Quan

作者机构：

关键词： Toxoplasma gondii; TgERK7; Eradication; Complemental expression; Invasion

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2020 年 119 卷 11 期

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收录情况： SCI

摘要： Toxoplasma gondiicauses serious clinical toxoplasmosis in humans mostly due to its asexual life cycles, which can be artificially divided into five tightly coterminous stages. Any radical or delay for the stage will result in tremendous changes immediately behind. We previously demonstrated thatTgERK7 is associated with the intracellular proliferation ofT. gondii, but during the process, other stages before were not meanwhile determined. To further clarify the function of ERK7 gene inT. gondii, the complemental strain of Delta TgERK7 tachyzoites created previously was engineered via electric transfection with the recombinant pUC/Tgerk7plasmid, named pUC/TgERK7 strain in this study, and was used together with Delta TgERK7 and wild-type GT1 strains to retrospect the phenotypic changes including invasion and attachment. The results showed thatTgERK7 protein can be re-expressed in the Delta TgERK7 tachyzoites and eradication of this protein leads to significantly lower invasion ofT. gondiiat 1 h and 2 h post-infection (P< 0.05), which is the key factor causing the following slow intracellular proliferation, in comparison with wild-type GT1 and pUC/TgERK7 parasites; noteworthily, at other early time points including 15 min for attachment assay was no statistical difference (P> 0.05). The data suggested that ERK7 protein inT. gondiiis an important virulence factor that participates in the invasion of this parasite.

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