Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein

文献类型： 外文期刊

第一作者： Ge, Shikun

作者： Ge, Shikun;Xu, Long;Li, Ben;Liu, Xiang;Zhang, Xiaoying;Ge, Shikun;Xu, Long;Zhang, Xiaoying;Zhang, Xiaoying;Zhong, Fagang;Zhang, Xiaoying

作者机构：

关键词： IgY-Technology; infectious animal diseases; virus diseases; parvovirus; phage display system

期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）

ISSN： 0928-4249

年卷期： 2020 年 51 卷 1 期

页码：

收录情况： SCI

摘要： Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2. The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed inE. colisystem. The titer of the primary scFv library reached to 1.5 x 10(6)pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 h. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.

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