Assessing the efficacy of a recombinant H9N2 avian influenza virus-inactivated vaccine

文献类型： 外文期刊

第一作者： Song, Cai Liang

作者： Song, Cai Liang;Liao, Zhi Hong;Shen, Yong;Wang, Huang;Lin, Wen Cheng;Li, Hongxin;Chen, Wei Guo;Xie, Qing Mei;Song, Cai Liang;Liao, Zhi Hong;Shen, Yong;Wang, Huang;Lin, Wen Cheng;Li, Hongxin;Chen, Wei Guo;Xie, Qing Mei;Song, Cai Liang;Liao, Zhi Hong;Shen, Yong;Wang, Huang;Lin, Wen Cheng;Li, Hongxin;Chen, Wei Guo;Xie, Qing Mei;Song, Cai Liang;Liao, Zhi Hong;Shen, Yong;Wang, Huang;Lin, Wen Cheng;Li, Hongxin;Chen, Wei Guo;Xie, Qing Mei;Lin, Wen Cheng;Li, Hongxin;Chen, Wei Guo;Xie, Qing Mei

作者机构：

关键词： H9N2; reverse genetics system; vaccine efficacy

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

ISSN：

年卷期： 2020 年 99 卷 9 期

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收录情况： SCI

摘要： The H9N2 avian influenza virus has been widely spread in poultry around the world. It is proved to the world that the avian influenza virus can directly infect human beings without any intermediate host adaptation in "1997 Hong Kong avian influenza case," which shows that the avian influenza virus not only causes significant losses to the poultry industry but also affects human health. In this study, we aimed to address the problem of low protection of avian H9N2 subtype influenza virus vaccine against H9N2 wild-type virus. We have rescued the H9.4.2.5 branched avian influenza virus isolated in South China by reverse genetics technology. We have recombined these virus (rHA/NA-GD37 and rHA/NA-GD38) which contain hemagglutinin and neuraminidase genes from the H9N2 avian influenza virus (MN064850 or MN064851) and 6 internal genes from the avian influenza virus (KY785906). We compared the biological properties of the virus for example virus proliferation, virus elution, thermostability, and pH stability. Then, we evaluated the immune effects between rHA/NA-GD37 and GD37, which show that the recombinant avian influenza virus-inactivated vaccine can stimulate chickens to produce higher antibody titers and produce little inflammatory response after the challenge. It is noticeable that the recombinant virus-inactivated vaccine had better immune impact than the wild-type inactivated vaccine. Generally speaking, this study provides a new virus strain for the development of a H9N2 vaccine.

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