Actinoplanes solisilvaesp.nov., Isolated from Birch Forest Soil

文献类型： 外文期刊

第一作者： Ma, Qingyun

作者： Ma, Qingyun;Zhang, Qi;Jiang, Xu;Kong, Delong;Han, Xiaoyan;Zhou, Yiqing;Ruan, Zhiyong;Zhang, Qi;Zhang, Wei;Xue, Huiying;Ruan, Zhiyong;Zhang, Yuqin;Zhang, Qi

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2020 年 77 卷 11 期

页码：

收录情况： SCI

摘要： A novel actinomycete, designated strain LAM7112(T), was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 degrees C (optimum: 35 degrees C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The isolate formed very scantily irregular sporangia containing motile spores on the substrate mycelium. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate was closely related to members of the familyMicromonosporaceae, with highest similarites toActinoplanes ferrugineusX-14695(T)(97.4%),Micromonospora zamorensisDSM 45600(T)(97.3%) andMicromonospora aurantiacaATCC 27029(T)(97.3%). In the phylogenetic trees, strain LAM7112(T)formed a stable phylogenetic subclade within the genusActinoplanes. The genomic DNA G + C content was 70.0 mol%. The major fatty acids (> 10%) were determined to be iso-C-16:0, anteiso-C(15:0)and anteiso-C-17:0. The predominant menaquinones were identified as MK-9 (H-2), MK-9 (H-4) and MK-9 (H-6). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The diagnostic amino acid of the cell wall peptidoglycan was determined to bemeso-diaminopimelic acid. The diagnostic sugars in cell hydrolysates were determined to be glucose and ribose. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7112(T)(= CGMCC 4.7580(T) = JCM 32512(T)) is proposed to represent the type strain of a novel species of the genusActinoplanes, for which the nameActinoplanes solisilvaeis proposed.

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