Extraction Optimization, Purification and Immunostimulatory Activity in vitro of Polyphenols from Apple (Malus domestica) Peel

文献类型： 外文期刊

第一作者： Riaz, Asad

作者： Riaz, Asad;Abdin, Mohamed;Yousaf, Khurram;Jabbar, Saqib;Abid, Muhammad;Hashim, Malik Muhammad;Riaz, Asad

作者机构：

关键词： Apple peel; extraction; immunostimulating activity; polyphenols; purification; RAW264.7

期刊名称：SAINS MALAYSIANA （ 影响因子：1.009； 五年影响因子：0.95 ）

ISSN： 0126-6039

年卷期： 2020 年 49 卷 7 期

页码：

收录情况： SCI

摘要： Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction o f polyphenols were optimized by the response surface methodology The optimized conditions determined as 32 min of extraction time, 29 degrees C of extraction temperature and ethanol concentration of 56% with a yield of 35.08 +/- 0.26 mg gallic acid equivalent/g dry weight. Six resins were used for the purification and D101 resin showed the highest ratio of desorption for polyphenols and further applied for dynamic adsorption/desorption test. Catechin, procyandin B, querecitin-3-galactoside and querecitin xyloside were identified in purified polyphenols by HPLC-MS/MS. Finally the immunostimulatory activity in vitro of purified polyphenols on RAW264.7 cell lines was evaluated The apple peel polyphenols exhibited a dose-dependent effect (p < 0.05) on the strongest propagation of RAW264.7 cells. The results demonstrate that apple peel is a valuable source of polyphenols and could be used as a natural immunostimulating agent for application in functional foods.

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