Identification and Functional Analysis of AopN, anAcidovorax CitrulliEffector that Induces Programmed Cell Death in Plants

文献类型： 外文期刊

第一作者： Zhang, Xiaoxiao

作者： Zhang, Xiaoxiao;Jiang, Jie;Yang, Linlin;Yang, Yuwen;Qiu, Dewen;Zhao, Tingchang;Zhao, Mei;Walcott, Ron;Yang, Shanshan

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关键词： AopN; programmed cell death; Acidovorax citrulli; plant pathogen; effector-triggered immunity

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 17 期

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收录情况： SCI

摘要： Bacterial fruit blotch (BFB), caused byAcidovorax citrulli, seriously affects watermelon and other cucurbit crops, resulting in significant economic losses. However, the pathogenicity mechanism ofA. citrulliis not well understood. Plant pathogenic bacteria often suppress the plant immune response by secreting effector proteins. Thus, identifyingA. citrullieffector proteins and determining their functions may improve our understanding of the underlying pathogenetic mechanisms. In this study, a novel effector, AopN, which is localized on the cell membrane ofNicotiana benthamiana,was identified. The functional analysis revealed that AopN significantly inhibited the flg22-induced reactive oxygen species burst. AopN induced a programmed cell death (PCD) response. Unlike its homologous protein, the ability of AopN to induce PCD was dependent on two motifs of unknown functions (including DUP4129 and Cpta_toxin), but was not dependent on LXXLL domain. More importantly, the virulence of theaopNmutant ofA. citrulliinN. benthamianasignificantly decreased, indicating that it was a core effector. Further analysis revealed that AopN interacted with watermelon ClHIPP and ClLTP, which responds toA. citrullistrain Aac5 infection at the transcription level. Collectively, these findings indicate that AopN suppresses plant immunity and activates the effector-triggered immunity pathway.

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