Over-expression of glutamine synthetase genes Gln1-3/Gln1-4 improved nitrogen assimilation and maize yields

文献类型： 外文期刊

第一作者： He, ChunMei

作者： He, ChunMei;Liu, ChunXiao;Liu, Qiang;Gao, XinXue;Zhang, JuRen;Wang, LiMing;Liu, TieShan;Li, Ning;Zhang, JuRen

作者机构：

关键词： nitrogen; glutamine synthetase; transgene; yield; Zea mays

期刊名称：MAYDICA （ 影响因子：0.542； 五年影响因子：0.605 ）

ISSN： 0025-6153

年卷期： 2014 年 59 卷 3 期

页码：

收录情况： SCI

摘要： In agriculture, certain fertilizers that contain nitrogen generally tend to provide the most macronutrients for plant growth and development. The cDNAs of Gln1-3 and Gln1-4 genes, encoding glutamine synthetase isoenzymes (GS1), were fused to the rice actin1 promoter and over-expressed in the inbred maize line DH9632 by Agrobacterium-mediated genetic transformation. PCR assays demonstrated the integration of these genes in six transgenic lines. Transcription of Gln1-3 or Gln1-4 in the transformants was also confirmed by semi-quantitative RT-PCR and qRT-PCR; the transgenic lines had significantly higher expression compared with wild type. Transgenic lines L2 and L7 expressed the most Gln1-3 and Gln1-4 mRNA, respectively, and had the most enzyme activity in leaves below the ear after pollination for 14 days. Over-expression of these two genes led to increased chlorophyll content and improved photosynthesis after 14 days. In addition, yield-related traits such as ear length, ear diameter, ear weight, grain weight per ear, and hundred-kernel weight were improved in the transgenic lines. The plot yield of transgenic L2 was increased by approximately 20%. These results suggest that overexpression of Gln1-3 and Gln1-4 in maize improves yields and enhances nitrogen using efficiency. Thus, transgenic lines overexpressing Gln1-3 or Gln1-4 in maize could potentially be used in maize breeding.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序