Lipid Dynamics, Identification, and Expression Patterns of Fatty Acid Synthase Genes in an Endoparasitoid,Meteorus pulchricornis (Hymenoptera: Braconidae)

文献类型： 外文期刊

第一作者： Wang, Jiao

作者： Wang, Jiao;Shen, Li-Wei;Xing, Xiao-Rong;Xie, Yu-Qi;Li, Yi-Jiangcheng;Liu, Zhi-Xiang;Wang, Jun;Wu, Fu-An;Sheng, Sheng;Wang, Jun;Wu, Fu-An;Sheng, Sheng

作者机构：

关键词： Meteorus pulchricornis; lipid content; fatty acid synthase genes; lipogenesis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 17 期

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收录情况： SCI

摘要： In insect parasitoids, fatty acid synthases (FASs) have received less attention and their roles associated with lipogenesis loss are far from clear.Meteorus pulchricornisis a solitary endoparasitoid wasp of many larvae of lepidopteran pests. The lipid content during developmental stages ofM. pulchricorniswas measured; it was higher in the larval and pupal stages but declined from six-day-old pupae. Lipid accumulation constantly decreased in the adult stage, even after feeding on honey solutions. To investigate the roles of FASs in lipid synthesis inM. pulchricornis, four FAS genes (MpulFAS1 similar to 4) were identified from the transcriptome database ofM. pulchricornis. All FAS genes included full-length open reading frames and shared 72-79% similarity with the sequences ofMicroplitis demolitor. qRT-PCR validation showed that all fourFASshad the highest expression after the adult wasps were fed on honey diets.MpulFAS1andMpulFAS2reached their expression peaks at the adult stage but pulFAS3 and MpulFAS4 peaked at the larval stage. To further study the function of FASs, dsRNA injection knocked down the expression of fourMpulFASsand resulted in a significant decline of lipid content at the adult stage inM. pulchricornis. Results from this study suggest thatM. pulchricornisadults cannot accumulate lipid content effectively and FASs may still contribute to lipid synthesis in the adult stage. This broadens the knowledge on the ability of lipid synthesis in parasitoid wasps and provides insight into the roles of FASs in insects with parasitic life-history traits.

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