DietaryBacillusspp.enhanced growth and disease resistance of weaned pigs by modulating intestinal microbiota and systemic immunity

文献类型： 外文期刊

第一作者： He, Yijie

作者： He, Yijie;Jinno, Cynthia;Kim, Kwangwook;Liu, Yanhong;Wu, Zhaohai;Tan, Bie;Li, Xunde;Whelan, Rose

作者机构：

关键词： Bacillusspp; Diarrhea; Escherichia coli; Gut health; Weaned pigs

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：5.032； 五年影响因子：5.922 ）

ISSN： 1674-9782

年卷期： 2020 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Background Previous research has shown that dietary supplementation ofBacillusspp. probiotics exerts beneficial effects on animals' growth. However, limited studies have evaluated the efficacy ofBacillusspp.on weaned pigs and their effects on host gut health and microbiome, and systemic immunity using a disease challenge model. The objective of this experiment was to investigate the effects of twoBacillusspp.strains (Bacillus subtilisDSM 32540 andBacillus pumilusDSM 32539) on growth performance, diarrhea, intestinal health, microbiome, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenicEscherichia coli(ETEC). Results Pigs in PRO1 (Bacillus subtilisDSM 32540) had greater (P < 0.05) body weight on d 7 and 14 PI, greater (P < 0.05) ADG from d 0 to 7 and d 7 to 14 PI, compared with pigs in CON (Control). Pigs in PRO1 had milder (P < 0.05) diarrhea on d 2 and 3 PI compared with pigs in CON. However, no differences were observed in growth performance and diarrhea score between PRO2 (Bacillus pumilusDSM 32539) and CON groups. Supplementation of PRO1 decreased (P < 0.05) lymphocyte counts on d 7 and 14 PI, compared with CON. Supplementation of PRO1 and PRO2 both reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO2 had greater (P < 0.05) goblet cell number and sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in CON. Supplementation of PRO1 up-regulated (P < 0.05)MUC2gene expression in jejunal mucosa and reduced (P < 0.05)PTGS-2andIL1Bgene expression in ileal mucosa on d 21 PI, compared with CON. Pigs in PRO1 had reduced (P < 0.05) relative abundance of families Lachnospiraceae, Peptostreptococcaceae and Pasteurellaceae in the ileum. Conclusions Supplementation ofBacillus subtilisDSM 32540 improved growth performance, alleviated diarrhea severity, enhanced gut health, and reduced systemic inflammation of weaned pigs infected with ETEC F18. AlthoughBacillus pumilusDSM 32539 was able to alleviate systemic inflammation, it had limited impacts on growth performance and severity of diarrhea of ETEC F18 challenged weaned pigs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序