Formyl tetrahydrofolate deformylase affects hydrogen peroxide accumulation and leaf senescence by regulating the folate status and redox homeostasis in rice

文献类型： 外文期刊

第一作者： Xiong, Erhui

作者： Xiong, Erhui;Chen, Fei;Zhang, Chen;Li, Shan;Zhang, Yanli;Wu, Limin;Yu, Yanchun;Dong, Guojun;Qian, Qian;Shohag, Jahidul Islam;Yang, Xiaoe;Zhou, Yihua;Xiong, Erhui

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关键词： H2O2; leaf senescence; tetrahydrofolate; oxidative phosphorylation; GSH; programmed cell death

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

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收录情况： SCI

摘要： It is well established that an abnormal tetrahydrofolate (THF) cycle causes the accumulation of hydrogen peroxide (H2O2) and leaf senescence, however, the molecular mechanism underlying this relationship remains largely unknown. Here, we reported a novel rice tetrahydrofolate cycle mutant, which exhibited H(2)O(2)accumulation and early leaf senescence phenotypes. Map-based cloning revealed thatHPA1encodes a tetrahydrofolate deformylase, and its deficiency led to the accumulation of tetrahydrofolate, 5-formyl tetrahydrofolate and 10-formyl tetrahydrofolate, in contrast, a decrease in 5,10-methenyl-tetrahydrofolate. The expression of tetrahydrofolate cycle-associated genes encoding serine hydroxymethyl transferase, glycine decarboxylase and 5-formyl tetrahydrofolate cycloligase was significantly down-regulated. In addition, the accumulation of H(2)O(2)inhpa1was not caused by elevated glycolate oxidation. Proteomics and enzyme activity analyses further revealed that mitochondria oxidative phosphorylation complex I and complex V were differentially expressed inhpa1, which was consistent with the H(2)O(2)accumulation inhpa1. In a further feeding assay with exogenous glutathione (GSH), a non-enzymatic antioxidant that consumes H2O2, the H(2)O(2)accumulation and leaf senescence phenotypes ofhpa1were obviously compensated. Taken together, our findings suggest that the accumulation of H(2)O(2)inhpa1may be mediated by an altered folate status and redox homeostasis, subsequently triggering leaf senescence.

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