Limnobacter alexandriisp. nov., a thiosulfate-oxidizing, heterotrophic and EPS-bearingBurkholderiaceaeisolated from cultivable phycosphere microbiota of toxicAlexandrium catenellaLZT09

文献类型： 外文期刊

第一作者： Duan, Yuhan

作者： Duan, Yuhan;Jiang, Zhiwei;Wu, Zheng;Sun, Jingya;Zhang, Xiaoling;Yang, Qiao;Jiang, Zhiwei;Yang, Xi;Sheng, Zhi;Yu, Xinwei;Yan, Jianbo

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关键词： Phycosphere microbiota; Limnobacter alexandriisp; nov; Alexandrium catenella; Toxic marine dinoflagellates; Bioactive exopolysaccharides; Thiosulfate-oxidizing

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2020 年 113 卷 11 期

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收录情况： SCI

摘要： A novel Gram-negative, aerobic, motile and short rod-shaped bacterium with exopolysaccharides production, designated as LZ-4(T), was isolated from cultivable phycosphere microbiota of harmful algal blooms-causing marine dinoflagellateAlexandrium catenellaLZT09 which produces paralytic shellfish poisoning toxins. Strain LZ-4(T)was able to use thiosulfate (optimum concentration 10 mM) as energy source for bacterial growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LZ-4(T)belonged to the genusLimnobacter, showing high 16S rRNA gene sequences similarities withL. thiooxidansDSM 13612(T)(99.4%),L. humiNBRC 11650(T)(98.2%) andL. litoralisNBRC 105857(T)(97.2%), respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between LZ-4(T)andL. thiooxidansDSM 13612(T)were 78.9 and 21.9%, respectively. Both values were far lower than the thresholds (95-96% for ANI and 70% for dDDH) generally accepted for new species delineation. The respiratory quinone of strain LZ-4(T)was Q-8. The dominant cellular fatty acids were determined as summed feature 3 (C-16:1 omega 6c/omega 7c), summed feature 8 (C-18:1 omega 6c/omega 7c) and C-16:0. Polar lipids profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified polar lipids. The genomic DNA G+C content of strain LZ-4(T)was 52.5 mol%. Based on polyphasic characterization, strain LZ-4(T)represents a novel species of the genusLimnobacter, for which the nameLimnobacter alexandriisp. nov. is proposed. The type strain is LZ-4(T)(=CCTCC AB 2019004(T) =KCTC 72281(T)).

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