Effect of ammonia on anaerobic digestion of municipal solid waste: Inhibitory performance, bioaugmentation and microbiome functional reconstruction

文献类型： 外文期刊

第一作者： Yan, Miao

作者： Yan, Miao;Tian, Hailin;Zhu, Xinyu;Khoshnevisan, Benyamin;Tsapekos, Panagiotis;Angelidaki, Irini;Fotidis, Ioannis A.;Treu, Laura;Campanaro, Stefano;Campanaro, Stefano;Tian, Hailin;Khoshnevisan, Benyamin;Fotidis, Ioannis A.

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关键词： Bioaugmentation; Metagenomics; Ammonia tolerance; Energy-converting mechanisms; Interspecies interaction; Metabolic reconstruction

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.273； 五年影响因子：11.529 ）

ISSN： 1385-8947

年卷期： 2020 年 401 卷

页码：

收录情况： SCI

摘要： The bioaugmentation is crucial to improve the energy-efficient process for anaerobic digestion of organic wastes at high ammonia levels. Genomic insights into the intricate microbial networks at a high ammonia level remain underexplored. The present study showed that the addition of Methanoculleus sp. DTU887 remarkably enhanced the methane production yield of organic fraction of municipal solid waste by 21% and decreased the volatile fatty acids by 10% when compared to the period before bioaugmentation. Genome-centric metagenomics reports the functional contribution of microbial members during organic waste degradation under the extremely high level of 13.5 g NH4+-N/L. Specifically, metabolic reconstruction revealed that these organisms have the potential to perform fermentative and acetogenic catabolism, a process facilitated by energy conservation-related with H-2/CO2 metabolism. Peptococcaceae spp. (DTU903, DTU900, and DTU895). and Tissierellales sp. DTU879 could degrade the organic waste hydrolysis product, i.e., glucose to acetate and H-2. Tissierellales sp. DTU879 and Syntrophaceticus sp. DTU783 could degrade the derived acetate. The H-2 scavenging Methanoculleus sp. DTU887 performs complementary metabolic reactions with Peptococcaceae spp., Tissierellales sp. and Syntrophaceticus sp., indicating syntrophic glucose and acetate degradation. This research offers the first insight that the key organisms form a syntrophy-supported food web in response to the bioaugmentation with ammonia tolerant methanogens performed in an AD system subjected to severe ammonia inhibition.

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