Three Paralogous R2R3-MYB Genes Contribute to Delphinidin-Related Anthocyanins Synthesis inPetunia hybrida

文献类型： 外文期刊

第一作者： Fu, Zhenzhu

作者： Fu, Zhenzhu;Jiang, Hui;Chao, Yacong;Dong, Xiaoyu;Yuan, Xin;Wang, Limin;Zhang, Jing;Xu, Menglan;Wang, Huijuan;Li, Yanmin;Gao, Jie;Zhang, Hechen;Chao, Yacong;Xu, Menglan;Gao, Jie

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关键词： Anthocyanin; Petunia; Regulation; Transcription factor

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

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收录情况： SCI

摘要： ThreeANTHOCYANIN SYNTHESIS REGULATORgenes (ASR1-3) that encode R2R3-MYB transcription factors were identified recently fromPetunia hybrida. In this study, we conducted additional experiments to characterize their specific function in the regulation of anthocyanin synthesis. The ASR1-3 proteins were localized in the nucleus. Analysis of their regulatory function by transient expression in the petunia corolla and yeast two-hybrid assays showed that the residues Arg at position 51 and Ala at position 102 in the N-terminal domain were essential for the regulatory function of the proteins, and the conserved domain at the C-terminal end was important for activation of the protein. RNA sequencing of overexpression (OE) and RNA-interference transgenic lines confirmed that the ASR proteins specifically induce structural genes of anthocyanin synthesis, including the early biosynthesis genesCHSj,F3H, andF3 ' 5 ' H-1, the late biosynthesis genesDFR,ANS,RT,MT,AT, andGT, and the anthocyanin-related glutathioneS-transferase geneAN9. In addition, a member of the detoxifying efflux carrier family, aDTX35-like gene, was upregulated by ASRs. Determination of anthocyanin/anthocyanidin contents inASR1-OE petunia lines revealed that ASR1 especially induced the flavonoid 3 ',5 '-hydroxylase, which was consistent with the abundance of dihydromyricetins and delphinidins, but not cyanidins. TheASRgenes were induced under high-intensity light and upregulated the expression ofMYBxandMYB27, thereby providing feedback repression of anthocyanin synthesis. An updated model is presented outlining the mechanisms underlying anthocyanin synthesis in petunia.

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